Journal article

Cardiac Repair With a Novel Population of Mesenchymal Stem Cells Resident in the Human Heart

Yuan Zhang, Priyadharshini Sivakumaran, Andrew E Newcomb, Damian Hernandez, Nicole Harris, Ramin Khanabdali, Guei-Sheung Liu, Darren J Kelly, Alice Pebay, Alex W Hewitt, Andrew Boyle, Richard Harvey, Wayne A Morrison, David A Elliott, Gregory J Dusting, Shiang Y Lim

STEM CELLS | WILEY-BLACKWELL | Published : 2015

Abstract

Cardiac resident stem cells (CRSCs) hold much promise to treat heart disease but this remains a controversial field. Here, we describe a novel population of CRSCs, which are positive for W8B2 antigen and were obtained from adult human atrial appendages. W8B2(+) CRSCs exhibit a spindle-shaped morphology, are clonogenic and capable of self-renewal. W8B2(+) CRSCs show high expression of mesenchymal but not hematopoietic nor endothelial markers. W8B2(+) CRSCs expressed GATA4, HAND2, and TBX5, but not C-KIT, SCA-1, NKX2.5, PDGFRα, ISL1, or WT1. W8B2(+) CRSCs can differentiate into cardiovascular lineages and secrete a range of cytokines implicated in angiogenesis, chemotaxis, inflammation, extrac..

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Grants

Awarded by National Health and Medical Research Council of Australia


Awarded by ARC Special Initiative in Stem Cell Science


Awarded by Foundation Leducq


Funding Acknowledgements

We thank Geraldine Mitchell and Aaron Dingle for advice on the in vitro angiogenesis assays, and Jason Palmer for assistance in histology. This work was supported by the National Health and Medical Research Council of Australia (NHMRC 1024817, 373705, and 573732), St. Vincent's Hospital (Melbourne) Research Endowment Fund, and CASS foundation. Alice Pebay is a Career Development Award Fellow of the NHMRC and an ARC Future Fellow, and Gregory Dusting is a Principal Research Fellow of NHMRC. Richard Harvey was supported by grants from the ARC Special Initiative in Stem Cell Science (SR110001002) and Foundation Leducq (13 CVD 01). Support is also provided by the Victorian State Government's Department of Innovation, Industry and Regional Development's Operational Infrastructure Support Program.