Journal article
Renal participation of myeloperoxidase in antineutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis
KM O'Sullivan, CY Lo, SA Summers, KD Elgass, PJ McMillan, A Longano, SL Ford, PY Gan, PG Kerr, AR Kitching, SR Holdsworth
Kidney International | Published : 2015
DOI: 10.1038/ki.2015.202
Abstract
Myeloperoxidase (MPO) is an important neutrophil lysosomal enzyme, a major autoantigen, and a potential mediator of tissue injury in MPO-ANCA-associated vasculitis (MPO-AAV) and glomerulonephritis. Here we examined MPO deposition in kidney biopsies from 47 patients with MPO-AAV. Leukocyte accumulation and fibrin deposition consistent with cell-mediated immunity was a major feature. Tubulointerstitial macrophage, CD4+ and CD8+ T-cell, and neutrophil numbers correlated with low presenting eGFR. MPO was not detected in kidneys from patients with minimal change or thin basement membrane disease, but was prominent in glomerular, periglomerular, and tubulointerstitial regions in MPO-AAV. Extracell..
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Funding Acknowledgements
This study was funded by a Project Grant from the National Health and Medical Research Council of Australia (606683). The 3D-SIM super-resolution microscopy imaging was performed at the Biological Optical Microscopy Platform, The University of Melbourne (www.microscopy.unimelb.edu.au). We thank Kevan Polkinghorne for statistical advice.