Journal article

Quantifying relative within-host replication fitness in influenza virus competition experiments

Stephen M Petrie, Jeff Butler, Ian G Barr, Jodie McVernon, Aeron C Hurt, James M McCaw



Through accumulation of genetic mutations in the neuraminidase gene, the influenza virus can become resistant to antiviral drugs such as oseltamivir. Quantifying the fitness of emergent drug-resistant influenza viruses, relative to contemporary circulating viruses, provides valuable information to complement existing efforts in the surveillance of drug-resistance. We have previously developed a co-infection based method for the assessment of the relative in vivo fitness of two competing viruses. We have also introduced a model of within-host co-infection dynamics that enables relative within-host fitness to be quantified in these competitive-mixtures experiments. The model assumed that fitne..

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Awarded by Victorian Life Sciences Computation Initiative (VLSCI) grant

Awarded by National Health and Medical Research Council

Awarded by Australian Research Council

Funding Acknowledgements

We thank Mathew Dafilis for his assistance with the implementation of an optimised ODE solver using the CVode package for MATLAB. We acknowledge the use of computing resources from the NeCTAR research cloud ( NeCTAR is an Australian Government project conducted as part of the Super Science initiative and financed by the Education Investment Fund. This research was supported by a Victorian Life Sciences Computation Initiative (VLSCI) grant (VR0274) on its Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government, Australia. We also acknowledge the use of computing resources from the High Performance Computing Cluster at the University of Melbourne. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza at the Peter Doherty Institute for Infection and Immunity is supported by the Australian Government Department of Health. Jodie McVemon was supported by a National Health and Medical Research Council Career Development Award (1061321). James McCaw was supported by an Australian Research Council Future Fellowship (110100250).