Journal article

Molecular detection of Cyclospora in water, soil, vegetables and humans in southern Italy signals a need for improved monitoring by health authorities

Annunziata Giangaspero, Marianna Marangi, Anson V Koehler, R Papini, G Normanno, V Lacasella, A Lonigro, Robin B Gasser

International Journal of Food Microbiology | ELSEVIER SCIENCE BV | Published : 2015

Abstract

To date, in Europe, there is scant information on the occurrence of Cyclospora in water from treatment plants and in humans, and no data are available on soil or fresh plant products. Here, we undertook the first molecular survey of Cyclospora in multiple biological matrices collected from the Apulia region of southern Italy. Samples of irrigation water from four municipal treatment plants, eight different types of vegetables or fruit (cucumber, lettuce, fennel, celery, tomato, melon, endive and chicory) and soil from the same farms on which these plants were grown, as well as faecal samples from humans living in the same region were tested by qPCR-coupled single-strand conformation polymorp..

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Grants

Awarded by In.Te.R.R.A.


Awarded by Victorian Life Sciences Computation Initiative (VLSCI)


Funding Acknowledgements

The authors thank Tiziana Caradonna and Raffaella Terlizzi for technical assistance. This study was part of the project "Technology and process innovations for irrigation re-use of treated municipal and agroindustrial wastewaters in order to achieve sustainable water resources management" (In.Te.R.R.A.,; grant number 01-01480), co-funded by the Italian Ministry of Universities and Research (MIUR), within the Italian "PON/Ricerca e Competitivita 2007-2013" Programme. RBG's research was supported by the Australian Research Council (ARC), the National Health 82 Medical Research Council (NHMRC) of Australia, Melbourne Water Corporation and Yourgene Bioscience; it was also supported by a Victorian Life Sciences Computation Initiative (VLSCI; grant number VR0007) on its Peak Computing Facility at the University of Melbourne, an initiative of the Victorian Government