Journal article

Safety of intracoronary human cord blood stem cells in a lamb model of infant cardiopulmonary bypass

CP Brizard, JYJ Looi, JJ Smolich, SB Horton, J Angerosa, NJ Elwood, S Pepe

Annals of Thoracic Surgery | Published : 2015

Abstract

Background One potential approach for advancing univentricular heart surgical palliation outcomes is by stem cell therapy to augment right ventricular function and muscle mass. Whether the stem cell-inclusive cord blood mononuclear cells (CBMNCs) are safe to perfuse into the coronary vasculature during neonatal cardiopulmonary bypass (CPB) is unknown. We evaluated the acute safety, functional effects, and fate of human CBMNCs in a novel model of coronary vasculature delivery in a lamb model of infant CPB. Methods Neonatal lambs were randomized in blinded fashion to receive control (n = 5) or human CD45+ CBMNCs (8 × 106 cells/kg body weight, n = 7) treatments during CPB. Aortic cross-clamp ti..

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