Journal article

Evaluation of biomarkers for in vitro prediction of druginduced nephrotoxicity: comparison of HK-2, immortalized human proximal tubule epithelial, and primary cultures of human proximal tubular cells

Johnny X Huang, Geraldine Kaeslin, Max V Ranall, Mark A Blaskovich, Bernd Becker, Mark S Butler, Melissa H Little, Lawrence H Lash, Matthew A Cooper

PHARMACOLOGY RESEARCH & PERSPECTIVES | JOHN WILEY & SONS LTD | Published : 2015

Abstract

There has been intensive effort to identify in vivo biomarkers that can be used to monitor drug-induced kidney damage and identify injury before significant impairment occurs. Kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL), and human macrophage colony stimulating factor (M-CSF) have been validated as urinary and plasma clinical biomarkers predictive of acute and chronic kidney injury and disease. Similar validation of a high throughput in vitro assay predictive of nephrotoxicity could potentially be implemented early in drug discovery lead optimization to reduce attrition at later stages of drug development. To assess these known in vivo biomarkers for th..

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Grants

Awarded by NHMRC


Awarded by NIH


Awarded by Wellcome Trust Seeding Drug Discovery Award


Awarded by NHMRC Professorial Research Fellowship


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

This research was supported financially by NHMRC grants APP1005350, APP1045326, and AF511105, and NIH grant 1R21AI098731 - 01. J. X. H., M. A. B. and M. S. B. were supported by a Wellcome Trust Seeding Drug Discovery Award 094977/Z/10/Z, and MAC by an NHMRC Professorial Research Fellowship (APP1059354).