Journal article

B cell-derived circulating granzyme B is a feature of acute infectious mononucleosis

Magdalena Hagn, Archana Panikkar, Corey Smith, Henry H Balfour, Rajiv Khanna, Ilia Voskoboinik, Joseph A Trapani

CLINICAL & TRANSLATIONAL IMMUNOLOGY | NATURE PUBLISHING GROUP | Published : 2015

Abstract

Granzyme B (GzmB) is a serine protease best known for inducing target cell apoptosis when released by cytotoxic T lymphocytes (CTLs) or natural killer cells with pore-forming perforin. As a result, GzmB detected in the serum of virus-infected individuals has typically been attributed to these sources. Here, we show that patients with recently diagnosed infectious mononucleosis caused by Epstein-Barr virus (EBV) have high circulating levels of GzmB that may be derived from infected B cells early in course of disease. We recently reported that human B cells from healthy donors secrete active GzmB when stimulated in vitro through B-cell receptor (BCR) ligation and interleukin (IL)-21. We found ..

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Grants

Awarded by Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)


Funding Acknowledgements

We thank Vicki Milovac, Sophie Kotsakidis and Ralph Rossi for flow cytometry support, and Amanda Ross for help with recruiting blood donation volunteers at Peter MacCallum Cancer Centre. We thank Dr Joanne Davis (Royal Melbourne Hospital, Australia) for B95.8 virus. This work was supported by Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) grant HA 6136/1-1 to MH, and by Program and Project Grants from the National Health and Medical Research Council of Australia to JAT and IV.