Journal article

The microbiome of otitis media with effusion in Indigenous Australian children

Jake Jervis-Bardy, Geraint B Rogers, Peter S Morris, Heidi C Smith-Vaughan, Elizabeth Nosworthy, Lex EX Leong, Renee J Smith, Laura S Weyrich, Jacques De Haan, A Simon Carney, Amanda J Leach, Stephen O'Leary, Robyn L Marsh

INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGOLOGY | ELSEVIER IRELAND LTD | Published : 2015

Abstract

INTRODUCTION: Indigenous Australian children have a high prevalence of otitis media with effusion (OME) and associated conductive hearing loss. Only three microbiological studies of middle ear fluid (MEF) from Indigenous Australian children with OME have been reported. All of these were reliant on culture or species-specific PCR assays. The aim of this study was to characterise the middle ear fluid (MEF), adenoid and nasopharyngeal (NP) microbiomes of Indigenous Australian children, using culture-independent 16S rRNA gene sequencing. METHODS: MEF, NP swabs and adenoid specimens were collected from 11 children in the Alice Springs region of Central Australia. Bacterial communities in these sp..

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University of Melbourne Researchers