Polarity Protein Scrib Facilitates Endothelial Inflammatory Signaling
Christoph Kruse, Angela RM Kurz, Katalin Palfi, Patrick O Humbert, Markus Sperandio, Ralf P Brandes, Christian Fork, U Ruth Michaelis
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY | LIPPINCOTT WILLIAMS & WILKINS | Published : 2015
OBJECTIVE: The polarity protein Scrib is highly expressed in endothelial cells and is required for planar cell polarity. Scrib also facilitates recycling of integrin α5 to the plasma membrane. Because integrin α5 signals the presence of the inflammatory matrix protein fibronectin, we hypothesized that Scrib contributes to endothelial inflammatory signaling. APPROACH AND RESULTS: Cytokine treatment of human umbilical vein endothelial cells induced an inflammatory response as evident by the induction of vascular cell adhesion molecule-1 (VCAM-1). Downregulation of Scrib greatly attenuated this effect. In endothelial-specific conditional Scrib knockout mice, in vivo lipopolysaccharide treatment..View full abstract
The study was supported by the DFG Excellence Cluster Cardiopulmonary System-ECCPS (U.R. Michaelis, R.P. Brandes), the SFB815 (TP A1) and SFB 834 (TPA2 to R.P. Brandes and TP A10 to U.R. Michaelis), and the DZHK (to R.P. Brandes and M. Sperandio). P.O. Humbert was supported by the Australian National Health and Medical Research Council of Australia.