High-Content Assay for Measuring Intracellular Growth of Leishmania in Human Macrophages
Michael J Dagley, Eleanor C Saunders, Kaylene J Simpson, Malcolm J McConville
ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES | MARY ANN LIEBERT, INC | Published : 2015
Leishmania species are sandfly-transmitted protozoan parasites that cause a spectrum of diseases, ranging from localized skin lesions to fatal visceral disease, in more than 12 million people worldwide. These parasites primarily target macrophages in their mammalian hosts and proliferate as non-motile amastigotes in the phagolysosomal compartment of these cells. High-throughput screens for measuring Leishmania growth within this intracellular niche are needed to identify host and parasite factors that are required for virulence and to identify new drug candidates. Here we describe the development of a new high-content imaging method for quantifying the intracellular growth of Leishmania mexi..View full abstract
Awarded by NHMRC
This work was supported by the NHMRC Research Project APP1059545. M.J.M. is an NHMRC Principal Research Fellow. The Victorian Centre for Functional Genomics (K.J.S.) is funded by the Australian Cancer Research Foundation (ACRF), the Victorian Department of Industry, Innovation and Regional Development (DIIRD), the Australian Phenomics Network (APN) and supported by funding from the Australian Government's Education Investment Fund through the Super Science Initiative, the Australasian Genomics Technologies Association (AMATA), the Brockhoff Foundation, and the Peter MacCallum Cancer Centre Foundation. The authors thank Daniel Thomas and Jennii Luu from the Victorian Centre for Functional Genomics for expert technical assistance.