Journal article
The ontogeny of naive and regulatory CD4 T-cell subsets during the first postnatal year: a cohort study
Fiona M Collier, Mimi Lk Tang, David Martino, Richard Saffery, John Carlin, Kim Jachno, Sarath Ranganathan, David Burgner, Katrina J Allen, Peter Vuillermin, Anne-Louise Ponsonby
CLINICAL & TRANSLATIONAL IMMUNOLOGY | NATURE PUBLISHING GROUP | Published : 2015
DOI: 10.1038/cti.2015.2
Open access
Abstract
As there is limited knowledge regarding the longitudinal development and early ontogeny of naïve and regulatory CD4(+) T-cell subsets during the first postnatal year, we sought to evaluate the changes in proportion of naïve (thymic and central) and regulatory (resting and activated) CD4(+) T-cell populations during the first postnatal year. Blood samples were collected and analyzed at birth, 6 and 12 months of age from a population-derived sample of 130 infants. The proportion of naïve and regulatory CD4(+) T-cell populations was determined by flow cytometry, and the thymic and central naïve populations were sorted and their phenotype confirmed by relative expression of T cell-receptor excis..
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Funding Acknowledgements
The funding for this work was totally supported by the National Health and Medical Research Council (NHMRC), Australia, and Barwon Health. We acknowledge scientists Danielle Kennedy and Carling Southall for their consistent and meticulous work in processing all the blood and mononuclear cell samples for flow cytometric analysis.