Journal article

Identification of a Potent Microbial Lipid Antigen for Diverse NKT Cells

Benjamin J Wolf, Raju VV Tatituri, Catarina F Almeida, Jerome Le Nours, Veemal Bhowruth, Darryl Johnson, Adam P Uldrich, Fong-Fu Hsu, Manfred Brigl, Gurdyal S Besra, Jamie Rossjohn, Dale I Godfrey, Michael B Brenner

JOURNAL OF IMMUNOLOGY | AMER ASSOC IMMUNOLOGISTS | Published : 2015

Abstract

Semi-invariant/type I NKT cells are a well-characterized CD1d-restricted T cell subset. The availability of potent Ags and tetramers for semi-invariant/type I NKT cells allowed this population to be extensively studied and revealed their central roles in infection, autoimmunity, and tumor immunity. In contrast, diverse/type II NKT (dNKT) cells are poorly understood because the lipid Ags that they recognize are largely unknown. We sought to identify dNKT cell lipid Ag(s) by interrogating a panel of dNKT mouse cell hybridomas with lipid extracts from the pathogen Listeria monocytogenes. We identified Listeria phosphatidylglycerol as a microbial Ag that was significantly more potent than a prev..

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Grants

Awarded by National Institutes of Health


Awarded by National Health and Medical Research Council of Australia


Awarded by Australian Research Council


Awarded by Medical Research Council


Awarded by Washington University Mass Spectrometry Resource


Awarded by Fundacao para a Ciencia e Tecnologia International PhD Programme from Ministerio da Educacao e Ciencia, Portugal


Awarded by National Health and Medical Research Council of Australia Senior Principal Research Fellowship


Awarded by Australian Research Council Future Fellowship


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Awarded by NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES


Awarded by NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES


Awarded by NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES


Funding Acknowledgements

This work was supported by National Institutes of Health Grants 5R01AI063428-09 and 5T32AR007530-30 (to the Brenner Laboratory), National Health and Medical Research Council of Australia Grants 1021972 and 1013667 (to the Godfrey Laboratory), and Australian Research Council Grant CE140100011. G.S.B. acknowledges support in the form of a Personal Research Chair from Mr. James Bardrick and the Medical Research Council (MR/K012118/1). F.-F.H. was supported by Washington University Mass Spectrometry Resource Grants P41-GM103422, P60-DK-20579, and P30-DK56341. M.B.B. was supported by National Institutes of Health Grants 5K08AI077795 and 1R21AI103616. C.F.A. was supported by Fundacao para a Ciencia e Tecnologia International PhD Programme SFRH/BD/74906/2010 from Ministerio da Educacao e Ciencia, Portugal. D.I.G. was supported by National Health and Medical Research Council of Australia Senior Principal Research Fellowship 1020770. J.R. was supported by a National Health and Medical Research Council of Australia Fellowship, the National Health and Medical Research Council of Australia, and the Australian Research Council. A.P.U. was supported by Australian Research Council Future Fellowship FT140100278.