Journal article

Analytical Bias in the Measurement of Serum 25-Hydroxyvitamin D Concentrations Impairs Assessment of Vitamin D Status in Clinical and Research Settings

Lucinda J Black, Denise Anderson, Michael W Clarke, Anne-Louise Ponsonby, Robyn M Lucas

PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2015

Abstract

Measured serum 25-hydroxyvitamin D concentrations vary depending on the type of assay used and the specific laboratory undertaking the analysis, impairing the accurate assessment of vitamin D status. We investigated differences in serum 25-hydroxyvitamin D concentrations measured at three laboratories (laboratories A and B using an assay based on liquid chromatography-tandem mass spectrometry and laboratory C using a DiaSorin Liaison assay), against a laboratory using an assay based on liquid chromatography-tandem mass spectrometry that is certified to the standard reference method developed by the National Institute of Standards and Technology and Ghent University (referred to as the 'certi..

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Grants

Awarded by National Multiple Sclerosis Society of the USA


Funding Acknowledgements

This study was supported by grants from the National Multiple Sclerosis Society of the USA (RG3364A1/2), the National Health and Medical Research Council (NHMRC) of Australia, and Multiple Sclerosis Research Australia. LJB and DA were supported by the Telethon Kids Institute; MWC was supported by Bioplatforms Australia (NCRIS) and the Western Australian Government Innovation Co-investment Program (ICP); A-LP and RML were supported by NHMRC Fellowships.