Analytical Bias in the Measurement of Serum 25-Hydroxyvitamin D Concentrations Impairs Assessment of Vitamin D Status in Clinical and Research Settings
Lucinda J Black, Denise Anderson, Michael W Clarke, Anne-Louise Ponsonby, Robyn M Lucas
PLoS One | PUBLIC LIBRARY SCIENCE | Published : 2015
Measured serum 25-hydroxyvitamin D concentrations vary depending on the type of assay used and the specific laboratory undertaking the analysis, impairing the accurate assessment of vitamin D status. We investigated differences in serum 25-hydroxyvitamin D concentrations measured at three laboratories (laboratories A and B using an assay based on liquid chromatography-tandem mass spectrometry and laboratory C using a DiaSorin Liaison assay), against a laboratory using an assay based on liquid chromatography-tandem mass spectrometry that is certified to the standard reference method developed by the National Institute of Standards and Technology and Ghent University (referred to as the 'certi..View full abstract
Awarded by National Multiple Sclerosis Society of the USA
This study was supported by grants from the National Multiple Sclerosis Society of the USA (RG3364A1/2), the National Health and Medical Research Council (NHMRC) of Australia, and Multiple Sclerosis Research Australia. LJB and DA were supported by the Telethon Kids Institute; MWC was supported by Bioplatforms Australia (NCRIS) and the Western Australian Government Innovation Co-investment Program (ICP); A-LP and RML were supported by NHMRC Fellowships.