Journal article

Conserved features in TamA enable interaction with TamB to drive the activity of the translocation and assembly module

Joel Selkrig, Matthew J Belousoff, Stephen J Headey, Eva Heinz, Takuya Shiota, Hsin-Hui Shen, Simone A Beckham, Rebecca S Bamert, Minh-Duy Phan, Mark A Schembri, Matthew CJ Wilce, Martin J Scanlon, Richard A Strugnell, Trevor Lithgow



The biogenesis of membranes from constituent proteins and lipids is a fundamental aspect of cell biology. In the case of proteins assembled into bacterial outer membranes, an overarching question concerns how the energy required for protein insertion and folding is accessed at this remote location of the cell. The translocation and assembly module (TAM) is a nanomachine that functions in outer membrane biogenesis and virulence in diverse bacterial pathogens. Here we demonstrate the interactions through which TamA and TamB subunits dock to bridge the periplasm, and unite the outer membrane aspects to the inner membrane of the bacterial cell. We show that specific functional features in TamA h..

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University of Melbourne Researchers


Awarded by NHMRC Program Grant

Awarded by NHMRC Project grant

Funding Acknowledgements

We thank Chaille Webb for comments on the manuscript and Denisse Leyton for reagents. This research was undertaken on the SAXS/WAXS beamline at the Australian Synchrotron and the QCM-D facility at CSIRO. This work was supported through an NHMRC Program Grant (606788, to TL and RAS) and an NHMRC Project grant (APP1042651 to MAS), and an ARC Super Science Grant (to TL and RAS). TL is an ARC Laureate Fellow, MAS is an ARC Future Fellow, HHS is an ARC Super Science Fellow, MJB is an NHMRC Biomedical Fellow, JS is a Long-Term EMBO (non-stipendiary) and EIPOD fellow and MCJW is an NHMRC Senior Research Fellow.