Journal article

A Novel Mouse Model for Stable Engraftment of a Human Immune System and Human Hepatocytes

Helene Strick-Marchand, Mathilde Dusseaux, Sylvie Darche, Nicholas D Huntington, Nicolas Legrand, Guillemette Masse-Ranson, Erwan Corcuff, James Ahodantin, Kees Weijer, Hergen Spits, Dina Kremsdorf, James P Di Santo

PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2015

Abstract

Hepatic infections by hepatitis B virus (HBV), hepatitis C virus (HCV) and Plasmodium parasites leading to acute or chronic diseases constitute a global health challenge. The species tropism of these hepatotropic pathogens is restricted to chimpanzees and humans, thus model systems to study their pathological mechanisms are severely limited. Although these pathogens infect hepatocytes, disease pathology is intimately related to the degree and quality of the immune response. As a first step to decipher the immune response to infected hepatocytes, we developed an animal model harboring both a human immune system (HIS) and human hepatocytes (HUHEP) in BALB/c Rag2-/- IL-2Rγc-/- NOD.sirpa uPAtg/t..

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Grants

Awarded by Bill & Melinda Gates foundation


Awarded by Agence Nationale de la recherche programme Emergence


Awarded by Agence Nationale de la recherche programme LABEX


Awarded by Agence Nationale deRecherches sur le Sida et les hepatites virales


Awarded by European Commission Seventh Framework Programme PathCO


Funding Acknowledgements

This study was supported by Bill & Melinda Gates foundation global health program grant # 37869. Agence Nationale de la recherche programme Emergence grant #ANR-11-EMMA-026. Agence Nationale de la recherche programme LABEX grant #ANR-10-LBX-73. Agence Nationale deRecherches sur le Sida et les hepatites virales grant #2013-105. European Commission Seventh Framework Programme PathCO grant #HEALTH-F3-2012-305578. AXENIS provided support in the form of salaries for authors Nicolas Legrand and Erwan Corcuff, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the 'author contributions' section.