Comparison of the infectivity of the laboratory strain AD169 and a clinical isolate of human cytomegalovirus to human smooth muscle cells

Abstract

The results displayed by human cytomegalovirus (HCMV) IE/E antigen expression and virus release into the supernatant at various times post infection with a clinical isolate (C3/p5) and AD169 laboratory strain of HCMV, illustrated differences in the biology of these viruses on the various cell lines. While AD169 strain was shown to infect fibroblasts efficiently, it showed a low infectivity profile to smooth muscle cells, whereas C3/p5 displayed comparable infectivity characteristics on both cell lines. Neither virus demonstrated propensity for infecting endothelial cells, although passaging of the C3/p5 for additional 11 passages in endothelial cells provided virus capable of infecting endot..