γ-Interferon counteracts interleukin-1α stimulated expression of urokinase-type plasminogen activator in human endothelial cells in vitro

Abstract

The effect of γ-interferon (γ-IFN) on the interleukin-1α (IL-1γ) induced stimulation of urokinasetype plasminogen activator (u-PA) expression in human foreskin microvascular endothelial cells (HFMEC) and in human umbilical vein endothelial cells (HFMEC) was investigated. When γ-IFN and IL-1α were added to the cells simultaneously, γ-IFN inhibited the IL-1α induced increase in uPA antigen production in both HFMEC and HUVEC in a dose dependent fashion, with a maximum inhibitory effect achieved between 2.0 and 20.0U/ml of γ-IFN. Pretreatment of HFMEC with γ-IFN for 1 hour before addition of IL-1α resulted in a significant reduction in u-PA synthesis. However, when HFMEC were pretreated for 8 ho..