Journal article

REGULATION OF PLASMINOGEN-ACTIVATOR PRODUCTION BY BONE-RESORBING HORMONES IN NORMAL AND MALIGNANT OSTEOBLASTS

JA HAMILTON, S LINGELBACH, NC PARTRIDGE, TJ MARTIN

ENDOCRINOLOGY | ENDOCRINE SOC | Published : 1985

Abstract

The plasminogen activator (PA) activity of clonal rat osteogenic sarcoma cell (phenotypically osteoblast) and of osteoblast-rich rat calvarial cells is shown to be increased by treatment with the bone-resorbing hormones, PTH, 1,25-dihydroxyvitamin D3, prostaglandin E2, and epidermal growth factor. Dose-dependent increases were observed, after a lag period of 4 to 8 h. Stimulated and control PA activities were inhibited by actinomycin D and cycloheximide but not by cytosine arabinoside. Glucocorticoid hormones prevented the hormone stimulation, but other steroids did not. Calcitonin had no effect either on basal or on hormone-treated PA activity. Isobutyl-methylxanthine alone increased PA act..

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