Journal article

Characterization of Kinase Target Phosphorylation Consensus Motifs Using Peptide SPOT Arrays

Genie C Leung, James M Murphy, Doug Briant, Frank Sicheri, M Cretich (ed.), M Chiari (ed.)

PEPTIDE MICROARRAYS: METHODS AND PROTOCOLS | HUMANA PRESS INC | Published : 2009

Abstract

The human proteome is known to contain >500 protein kinases, which regulate almost all facets of cellular biology by the post-translational attachment of a phosphate moiety to serine, threonine, or tyrosine residues within a substrate protein. Most protein kinases remain poorly characterized and, as a result, current studies are directed toward defining their target substrates experimentally to gain a comprehensive view of the signaling proteins and pathways modulated by these kinases. Herein, we describe a rapid and convenient method for elucidating the consensus substrate motif for phosphorylation by a protein kinase using peptide SPOT arrays that are custom-synthesized on a cellulose memb..

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University of Melbourne Researchers