Contrast-enhanced ultrasound evaluation of renal microcirculation in sheep.
Antoine G Schneider, Paolo Calzavacca, Anthony Schelleman, Tim Huynh, Michael Bailey, Clive May, Rinaldo Bellomo
Intensive Care Med Exp | Published : 2014
BACKGROUND: Contrast-enhanced ultrasonography (CEUS) is a novel imaging modality to estimate microvascular perfusion. We aimed to assess renal cortical microcirculatory changes by CEUS during pharmacologically or mechanically induced modifications of renal blood flow (RBF) in experimental animals. METHODS: We implanted invasive transit-time Doppler flow probes and a vascular occluder around the renal artery in six Merino sheep. After induction of general anaesthesia, renal CEUS studies with destruction-replenishment sequences were performed at baseline and after different interventions aimed at modifying RBF. First, we administered angiotensin II (AngII) to achieve a 25% (AngII 25%) and 50% ..View full abstract