Journal article

Evidence of apoptosis induced by viral protein 2 of chicken anaemia virus

Amir Kaffashi, Charles N Pagel, Amir H Noormohammadi, Glenn F Browning

Archives of Virology | SPRINGER WIEN | Published : 2015


Although viral protein 3 (VP3) of chicken anaemia virus (CAV) has been well recognised as an inducer of apoptosis, viral protein 2 (VP2) of the virus has only been speculated to have apoptotic activity. This has not been verified because the open reading frame (ORF) encoding VP2 completely encompasses that encoding VP3, and thus the possibility of expression of VP3 cannot be excluded. The aim of this study was to elucidate the potential role of VP2 as an inducer of apoptosis. Site-directed mutagenesis was used to generate a point mutation that knocked out VP3 by early termination of its translation with a stop codon without imposing any change in the amino acid sequence of VP2. The mutated s..

View full abstract