Prion protein "gamma-cleavage": characterizing a novel endoproteolytic processing event
Victoria Lewis, Vanessa A Johanssen, Peter J Crouch, Genevieve M Klug, Nigel M Hooper, Steven J Collins
CELLULAR AND MOLECULAR LIFE SCIENCES | SPRINGER BASEL AG | Published : 2016
The cellular prion protein (PrP(C)) is a ubiquitously expressed protein of currently unresolved but potentially diverse function. Of putative relevance to normal biological activity, PrP(C) is recognized to undergo both α- and β-endoproteolysis, producing the cleavage fragment pairs N1/C1 and N2/C2, respectively. Experimental evidence suggests the likelihood that these processing events serve differing cellular needs. Through the engineering of a C-terminal c-myc tag onto murine PrP(C), as well as the selective use of a far-C-terminal anti-PrP antibody, we have identified a new PrP(C) fragment, nominally 'C3', and elaborating existing nomenclature, 'γ-cleavage' as the responsible proteolysis..View full abstract
Related Projects (6)
DETERMINING NORMAL PRION PROTEIN FUNCTION, EMPHASISING THE ROLE PLAYED BY PROTEIN CLEAVAGE, AND ALSO DEFINING THE RELATIONSHIP BETWEEN SURVEILLANCE INTENSITY AND ANNUAL SPORADIC CJD INCIDENCE
As a neurologist undertaking research into prion diseases over an extended period, I have been able to lead and participate in many projects..
Awarded by Australian Government National Health and Medical Research Council (NHMRC)
Awarded by Medical Research Council of Great Britain
Awarded by Medical Research Council
The authors thank Associate Professor Victoria Lawson for her kind gift of the RK13 cells, Professor Charles Weissmann for the Tga20 mice, and the Victorian Brain Bank Network for their assistance in human control brain tissue sampling. This work was supported by an Australian Government National Health and Medical Research Council (NHMRC) Program Grant #628946 (SJC, VL, VJ), Practitioner Fellowship #1005816 (SJC), Training Fellowship #567123 (VL), R. D. Wright Fellowship (CDF2) (PJC) and Project Grant #1061550 (PJC), a University of Melbourne Early Career Researcher Fellowship (VL), the CJD Support Group Network (VL), and the Medical Research Council of Great Britain (G0802189) (NMH).