Overexpression of ARBORKNOX1 delays the differentiation of induced somatic sector analysis (ISSA) derived xylem fibre cells in poplar stems
Emma Melder, Gerd Bossinger, Antanas V Spokevicius
TREE GENETICS & GENOMES | SPRINGER HEIDELBERG | Published : 2015
ARBORKNOX1 (ARK1), a poplar functional homolog of the Arabidopsis Class-I KNOTTED- like homeobox (KNOXI) transcription factor SHOOT MERISTEMLESS (STM), has been shown to have a role in maintaining meristematic cells in an undifferentiated state. Studies in which this gene was ectopically overexpressed identified a delay in the differentiation of cambial derivatives. Overexpression of ARK1 in poplar stem sectors, using the induced somatic sector analysis (ISSA) experimental system, was undertaken to further investigate its role in the differentiation of xylem fibre cells. ISSA involves the in vivo transformation of a small number of cambial cells in the stem with a gene of interest giving ris..View full abstract
The authors would like to thank Hossein Valipour Kahrood for valuable and insightful feedback on the manuscript and Dr Andrew Groover for kindly providing the ARK1 gene. E. Melder was funded through an Australian Postgraduate Award through the Australian Research Council.