Journal article
RNA sequencing supports distinct reactive oxygen species-mediated pathways of apoptosis by high and low size mass fractions of Bay leaf (Lauris nobilis) in HT-29 cells
AL Rodd, K Ververis, D Sayakkarage, AW Khan, H Rafehi, M Ziemann, SJ Loveridge, R Lazarus, C Kerr, T Lockett, A El-Osta, TC Karagiannis, LE Bennett
Food and Function | ROYAL SOC CHEMISTRY | Published : 2015
DOI: 10.1039/c5fo00467e
Abstract
Anti-proliferative and pro-apoptotic effects of Bay leaf (Laurus nobilis) in mammalian cancer and HT-29 adenocarcinoma cells have been previously attributed to effects of polyphenolic and essential oil chemical species. Recently, we demonstrated differentiated growth-regulating effects of high (HFBL) versus low molecular mass (LFBL) aqueous fractions of bay leaf and now confirm by comparative effects on gene expression, that HFBL and LFBL suppress HT-29 growth by distinct mechanisms. Induction of intra-cellular lesions including DNA strand breakage by extra-cellular HFBL, invoked the hypothesis that iron-mediated reactive oxygen species with capacity to penetrate cell membrane, were responsi..
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Funding Acknowledgements
The support of the Australian Institute of Nuclear Science and Engineering is acknowledged. TCK was the recipient of AINSE awards and ALR is the recipient of an AINSE PGRA. Epigenomic Medicine Laboratory is supported by an Australian Research Council Future Fellowship and McCord Research (the parental company of Pinnaclife, Inc). Supported in part by the Victorian Government's Operational Infrastructure Support Program. The authors would like to acknowledge the use of the facilities provided by Monash Micro Imaging @ AMREP and particularly, the expert assistance from Drs Stephen Cody and Iska Carmichael and to thank Katherine Robinson and Hema Jegasothy for preparing processed and size-fractionated bay leaf products.