Time-Resolved Emission Spectroscopy of the Dansyl Fluorescence Probe

Abstract

Using a cavity-dumped laser system as the light source, we have recorded time-resolved fluorescence spectra for the dansyl group in a variety of systems. The fluorescence spectrum of dansylphosphatidylethanolamine incorporated into a variety of lipid bilayer systems at ambient temperatures shows very marked time dependence, with red shifts of up to 40 nm over a 40-ns period. Similarly, large red shifts are observed for dansylamide in 1-butanol at low temperatures, which can be tentatively attributed to the initial rapid for mation of an intramolecular charge transfer state, followed by reorientation of the polar solvent molecules. The timeresolved behavior in the lipid bilayer system can, by..