Journal article

Laser capture microdissection as a tool to evaluate human papillomavirus genotyping and methylation as biomarkers of persistence and progression of anal lesions

Alyssa M Cornall, Jennifer M Roberts, Monica Molano, Dorothy A Machalek, Samuel Phillips, Richard J Hillman, Andrew E Grulich, Fengyi Jin, I Mary Poynten, David J Templeton, Suzanne M Garland, Sepehr N Tabrizi

BMJ OPEN | BMJ PUBLISHING GROUP | Published : 2015

Abstract

INTRODUCTION: Anal squamous cell carcinoma is preceded by persistent infection with high-risk human papillomavirus (HPV) and the cancer precursor, high-grade squamous intraepithelial lesion (HSIL). Detection of specific HPV genotypes and HPV-related biomarkers may be an option for primary anal screening. However, more data on the natural history of HPV-related anal lesions are required. The outcomes from this study will enhance our understanding of the clinical and biological behaviour of HPV-related anal lesions and inform the development of future HPV genotype and/or biomarker screening tests. METHODS AND ANALYSIS: HIV-negative and HIV-positive men who have sex with men, aged 35 years and ..

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