Applying advanced imaging techniques to a murine model of orthotopic osteosarcoma
Matthew L Broadhead, Zerina Lokmic, Mei Lin Tan, Andrew Stevenson, David S Binns, Carleen Cullinane, Rodney J Hicks, Peter FM Choong, Damian E Myers
Frontiers in Surgery | FRONTIERS MEDIA SA | Published : 2015
INTRODUCTION: Reliable animal models are required to evaluate novel treatments for osteosarcoma. In this study, the aim was to implement advanced imaging techniques in a murine model of orthotopic osteosarcoma to improve disease modeling and the assessment of primary and metastatic disease. MATERIALS AND METHODS: Intra-tibial injection of luciferase-tagged OPGR80 murine osteosarcoma cells was performed in Balb/c nude mice. Treatment agent [pigment epithelium-derived factor (PEDF)] was delivered to the peritoneal cavity. Primary tumors and metastases were evaluated by in vivo bioluminescent assays, micro-computed tomography, [(18)F]-Fluoride-PET and [(18)F]-FDG-PET. RESULTS: [(18)F]-Fluoride-..View full abstract
This work was funded by a Xavier Krikori Sarcoma Grant through the Australian Sarcoma Study Group (ASSG) and through a CASS Foundation grant (Australia). Dr. MB was supported by a National Health and Medical Research Council (NHMRC) Medical Postgraduate Scholarship.