Spatiotemporally Distinct Interactions with Dendritic Cell Subsets Facilitates CD4( ) and CD8( ) T Cell Activation to Localized Viral Infection
Jyh Liang Hor, Paul G Whitney, Ali Zaid, Andrew G Brooks, William R Heath, Scott N Mueller
IMMUNITY | CELL PRESS | Published : 2015
The dynamics of when and where CD4(+) T cells provide help for CD8(+) T cell priming and which dendritic cells (DCs) activate CD4(+) T cells in vivo after localized infection are poorly understood. By using a cutaneous herpes simplex virus infection model combined with intravital 2-photon imaging of the draining lymph node (LN) to concurrently visualize pathogen-specific CD4(+) and CD8(+) T cells, we found that early priming of CD4(+) T cells involved clustering with migratory skin DCs. CD8(+) T cells did not interact with migratory DCs and their activation was delayed, requiring later clustering interactions with LN-resident XCR1(+) DCs. CD4(+) T cells interacted with these late CD8(+) T ce..View full abstract
We thank S. Bedoui for discussions and C. Jones, G. Davey, and M. Damtsis for technical assistance. Supported by the National Health and Medical Research Council of Australia (P.G.W., A.G.B., W.R.H., and S.N.M.) and the Australian Research Council (W.R.H. and S.N.M.).