Enhanced Tissue Differentiation in the Developing Mouse Brain Using Magnetic Resonance Micro-Histology
Francesca C Norris, Joanne Betts-Henderson, Jack A Wells, Jon O Cleary, Bernard M Siow, Simon Walker-Samuel, Karen McCue, Paolo Salomoni, Peter J Scambler, Mark F Lythgoe
Magnetic Resonance in Medicine | WILEY | Published : 2013
PURPOSE: Worldwide efforts to understand developmental processes demand new high-resolution 3D imaging methods to detect the consequences of gene function in embryo development and diseases. Encouragingly, recent studies have shown that MRI contrast agents can highlight specific tissue structures in ex vivo adult mouse brains. MR imaging of mouse embryos is currently limited by a lack of tissue staining capabilities that would provide the flexibility and specificity offered by histological stains conventionally used for mouse embryo phenotyping. METHODS: The MRI staining properties of two readily available contrast agents, Mn-DPDP and Gd-DTPA, were investigated in mid-gestation mouse embryos..View full abstract
Grant sponsors: Engineering and Physical Sciences Research Council, the Biotechnology and Biological Sciences Research Council, the British Heart Foundation.