In vivo photoacoustic imaging of mouse embryos
Jan Laufer, Francesca Norris, Jon Cleary, Edward Zhang, Bradley Treeby, Ben Cox, Peter Johnson, Pete Scambler, Mark Lythgoe, Paul Beard
JOURNAL OF BIOMEDICAL OPTICS | SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS | Published : 2012
The ability to noninvasively image embryonic vascular anatomy in mouse models is an important requirement for characterizing the development of the normal cardiovascular system and malformations in the heart and vascular supply. Photoacoustic imaging, which can provide high resolution non invasive images of the vasculature based upon optical absorption by endogenous hemoglobin, is well suited to this application. In this study, photoacoustic images of mouse embryos were obtained ex vivo and in vivo. The images show intricate details of the embryonic vascular system to depths of up to 10 mm, which allowed whole embryos to be imaged in situ. To achieve this, an all-optical photoacoustic scanne..View full abstract
Awarded by British Heart Foundation
Awarded by Engineering and Physical Sciences Research Council
We would like to acknowledge the support of the British Heart Foundation and the Engineering and Physical Sciences Research Council.