When apoptotic cell death goes wrong, the results are often cancer or autoimmune diseases. My laboratory aims to illuminate critical "black boxes" in the apoptotic pathway.
We are focusing on how the killer Bak and Bax proteins irreversibly commit cells to die, and how these proteins are regulated. Understanding this process is critical to developing new treatments that either enhance or block apoptosis in diseased cells.
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Avoiding adsorption of Bcl-2 proteins to plasticware is important for accurate quantitatio..
Probing BAK and BAX Activation and Pore Assembly with Cytochrome c Release, Limited Proteo..
Mcl-1 and Bcl-x(L) sequestration of Bak confers differential resistance to BH3-only protei..
Displaying the 69 most recent scholarly works by Ruth Kluck.
Probing BAK and BAX Activation and Pore Assembly with Cytochrome c Release, Limited Proteolysis, and Oxidant-Induced Linkage.
Sweta Iyer, Rachel T Uren, Ruth M Kluck
Journal article | 2019 | Methods in molecular biology (Clifton, N.J.)
Mitochondrial permeabilization is a key event in the intrinsic pathway of apoptosis, and is mediated by either of the BCL-2 family..
VDAC2 enables BAX to mediate apoptosis and limit tumor development
Hui San Chin, Mark X Li, Iris KL Tan, Robert L Ninnis, Boris Reljic, Kristen Scicluna, Laura F Dagley, Jarrod J Sandow, Gemma L Kelly, Andre L Samson, Stephane Chappaz, Seong L Khaw, Catherine Chang, Andrew Morokoff, Kerstin Brinkmann, Andrew Webb, Colin Hockings, Cathrine M Hall, Andrew J Kueh, Michael T Ryan, et al.
Journal article | 2018 | Nature Communications
BAX requires VDAC2 to mediate apoptosis and to limit tumor development
Hui San Chin, Mark van Delft, Robert Ninnis, Mark Li, Iris Tan, Boris Reljic, Kristen Scicluna, Laura Dagley, Jarrod Sandow, Gemma Kelly, Stephane Chappaz, Seong Khaw, Catherine Chang, Andrew Webb, Colin Hockings, Cathrine Hall, Andrew Kueh, Michael Ryan, Ruth Kluck, Philippe Bouillet, et al.
Journal article | 2018
Abstract Intrinsic apoptosis is critical for normal physiology including the prevention of tumor formation. BAX and BAK are essent..
BAK alpha 6 permits activation by BH3-only proteins and homooligomerization via the canonical hydrophobic groove
Mark Xiang Li, Iris KL Tan, Stephen B Ma, Colin Hockings, Tobias Kratina, Michael A Dengler, Amber E Alsop, Ruth M Kluck, Grant Dewson
Journal article | 2017 | Proceedings of the National Academy of Sciences
Epigenetic control of mitochondrial cell death through PACS1-mediated regulation of BAX/BAK oligomerization
Daniella Brasacchio, Amber E Alsop, Tahereh Noori, Mariam Lufti, Sweta Iyer, Kaylene J Simpson, Phillip I Bird, Ruth M Kluck, Ricky W Johnstone, Joseph A Trapani
Journal article | 2017 | Cell Death & Differentiation
Disordered clusters of Bak dimers rupture mitochondria during apoptosis
Rachel Uren, Martin O’Hely, Sweta Iyer, Ray Bartolo, Jason Brouwer, Amber Alsop, Grant Dewson, Ruth Kluck
Journal article | 2016
ABSTRACT During apoptosis, Bak and Bax undergo major conformational change and form symmetric dimers that coalesce to perforate th..
Physiological restraint of Bak by Bcl-x(L) is essential for cell survival
Erinna F Lee, Stephanie Grabow, Stephane Chappaz, Grant Dewson, Colin Hockings, Ruth M Kluck, Marlyse A Debrincat, Daniel H Gray, Matthew T Witkowski, Marco Evangelista, Anne Pettikiriarachchi, Philippe Bouillet, Rachael M Lane, Peter E Czabotar, Peter M Colman, Brian J Smith, Benjamin T Kile, W Douglas Fairlie
Journal article | 2016 | Genes & Development
Bid chimeras indicate that most BH3-only proteins can directly activate Bak and Bax, and show no preference for Bak versus Bax
C Hockings, K Anwari, RL Ninnis, J Brouwer, M O'Hely, M Evangelista, MG Hinds, PE Czabotar, EF Lee, WD Fairlie, G Dewson, RM Kluck
Journal article | 2015 | Cell Death & Disease
Bax targets mitochondria by distinct mechanisms before or during apoptotic cell death: a requirement for VDAC2 or Bak for efficient Bax apoptotic function
SB Ma, TN Nguyen, I Tan, R Ninnis, S Iyer, DA Stroud, M Menard, RM Kluck, MT Ryan, G Dewson
Journal article | 2014 | Cell Death & Differentiation
Apoptotic pore formation is associated with in-plane insertion of Bak or Bax central helices into the mitochondrial outer membrane
Dana Westphal, Grant Dewson, Marie Menard, Paul Frederick, Sweta Iyer, Ray Bartolo, Leonie Gibson, Peter E Czabotar, Brian J Smith, Jerry M Adams, Ruth M Kluck
Journal article | 2014 | Proceedings of the National Academy of Sciences
Assembly of the Bak Apoptotic Pore A CRITICAL ROLE FOR THE BAK PROTEIN alpha 6 HELIX IN THE MULTIMERIZATION OF HOMODIMERS DURING APOPTOSIS
Stephen Ma, Colin Hockings, Khatira Anwari, Tobias Kratina, Stephanie Fennell, Michael Lazarou, Michael T Ryan, Ruth M Kluck, Grant Dewson
Journal article | 2013 | Journal of Biological Chemistry
Bax Crystal Structures Reveal How BH3 Domains Activate Bax and Nucleate Its Oligomerization to Induce Apoptosis
Peter E Czabotar, Dana Westphal, Grant Dewson, Stephen Ma, Colin Hockings, W Douglas Fairlie, Erinna F Lee, Shenggen Yao, Adeline Y Robin, Brian J Smith, David CS Huang, Ruth M Kluck, Jerry M Adams, Peter M Colman
Journal article | 2013 | Cell
Granzyme B triggers a prolonged pressure to die in Bcl-2 overexpressing cells, defining a window of opportunity for effective treatment with ABT-737
VR Sutton, K Sedelies, G Dewson, ME Christensen, PI Bird, RW Johnstone, RM Kluck, JA Trapani, NJ Waterhouse
Journal article | 2012 | Cell Death & Disease
A cytochrome c mutant with high electron transfer and antioxidant activities but devoid of apoptogenic effect
ZK Abdullaev, ME Bodrova, BV Chernyak, DA Dolgikh, RM Kluck, MO Perverzev, AS Arseniev, RG Efremov, MP Kirpichnikov, EN Mokhova, DD Newmeyer, H Roder, VP Skulachev
Journal article | 2002 | Biochemical Journal
A distinct pathway of cell-mediated apoptosis initiated by granulysin
AA Kaspar, S Okada, J Kumar, FR Poulain, KA Drouvalakis, A Kelekar, DA Hanson, RM Kluck, Y Hitoshi, DE Johnson, CJ Froelich, CB Thompson, DD Newmeyer, A Anel, C Clayberger, AM Krensky
Journal article | 2001 | Journal of Immunology
The pro-apoptotic proteins, Bid and Bax, cause a limited permeabilization of the mitochondrial outer membrane that is enhanced by cytosol
RM Kluck, MD Esposti, G Perkins, C Renken, T Kuwana, E Bossy-Wetzel, M Goldberg, T Allen, MJ Barber, DR Green, DD Newmeyer
Journal article | 1999 | Journal of Cell Biology
Ordering the cytochrome c-initiated caspase cascade: Hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner
EA Slee, MT Harte, RM Kluck, BB Wolf, CA Casiano, DD Newmeyer, HG Wang, JC Reed, DW Nicholson, ES Alnemri, DR Green, SJ Martin
Journal article | 1999 | Journal of Cell Biology
IMPAIRED BILIARY ELIMINATION OF BETA-GLUCURONIDASE-RESISTANT GLUCURONIDES OF VALPROIC ACID AFTER INTRAVENOUS ADMINISTRATION IN THE RAT - EVIDENCE FOR OXIDATIVE-METABOLISM OF THE RESISTANT ISOMERS
RG DICKINSON, RM KLUCK, BT WOOD, MJ EADIE, WD HOOPER
Journal article | 1986 | Drug Metabolism and Disposition
DISPOSITION OF BETA-GLUCURONIDASE-RESISTANT GLUCURONIDES OF VALPROIC ACID AFTER INTRABILIARY ADMINISTRATION IN THE RAT - INTACT ABSORPTION, FECAL EXCRETION AND INTESTINAL HYDROLYSIS
RG DICKINSON, RM KLUCK, MJ EADIE, WD HOOPER
Journal article | 1985 | Journal of Pharmacology and Experimental Therapeutics