John Silke

MLKL deficiency protects against low-grade, sterile inflammation in aged mice

Emma C Tovey C Crutchfield, Sarah E Garnish, Jessica Day, Holly Anderton, Shene Chiou, Anne Hempel, Cathrine Hall, Komal M Patel, Pradnya Gangatirkar, Katherine R Martin, Connie SN Li Wai Suen, Alexandra L Garnham, Andrew J Kueh, Ian P Wicks, John Silke, Ueli Nachbur, Andre L Samson, James M Murphy, Joanne M Hildebrand

Journal article  |  2023  |  CELL DEATH AND DIFFERENTIATION

MLKL and RIPK3 are the core signaling proteins of the inflammatory cell death pathway, necroptosis, which is a known mediator and ..

Caspase-8-driven apoptotic and pyroptotic crosstalk causes cell death and IL-1 beta release in X-linked inhibitor of apoptosis (XIAP) deficiency

Sebastian A Hughes, Meng Lin, Ashley Weir, Bing Huang, Liya Xiong, Ngee Kiat Chua, Jiyi Pang, Jascinta P Santavanond, Rochelle Tixeira, Marcel Doerflinger, Yexuan Deng, Chien-Hsiung Yu, Natasha Silke, Stephanie A Conos, Daniel Frank, Daniel S Simpson, James M Murphy, Kate E Lawlor, Jaclyn S Pearson, John Silke, et al.

Journal article  |  2023  |  EMBO JOURNAL

Genetic lesions in X-linked inhibitor of apoptosis (XIAP) pre-dispose humans to cell death-associated inflammatory diseases, altho..

The unifying catalytic mechanism of the RING-between-RING E3 ubiquitin ligase family.

Xiangyi S Wang, Thomas R Cotton, Sarah J Trevelyan, Lachlan W Richardson, Wei Ting Lee, John Silke, Bernhard C Lechtenberg

Journal article  |  2023  |  Nat Commun

The RING-between-RING (RBR) E3 ubiquitin ligase family in humans comprises 14 members and is defined by a two-step catalytic mecha..

OXTRHigh stroma fibroblasts control the invasion pattern of oral squamous cell carcinoma via ERK5 signaling.

Liang Ding, Yong Fu, Nisha Zhu, Mengxiang Zhao, Zhuang Ding, Xiaoxin Zhang, Yuxian Song, Yue Jing, Qian Zhang, Sheng Chen, Xiaofeng Huang, Lorraine A O'Reilly, John Silke, Qingang Hu, Yanhong Ni

Journal article  |  2022  |  Nat Commun

The Pattern Of Invasion (POI) of tumor cells into adjacent normal tissues clinically predicts postoperative tumor metastasis/recur..

Langerhans cells are an essential cellular intermediary in chronic dermatitis

Holly Anderton, Michael Chopin, Caleb A Dawson, Stephen L Nutt, Lachlan Whitehead, Natasha Silke, Najoua Lalaloui, John Silke

Journal article  |  2022  |  CELL REPORTS

SHARPIN regulates signaling from the tumor necrosis factor (TNF) superfamily and pattern-recognition receptors. An inactivating Sh..

Tankyrase-mediated ADP-ribosylation is a regulator of TNF-induced death

Lin Liu, Jarrod J Sandow, Deena M Leslie Pedrioli, Andre L Samson, Natasha Silke, Tobias Kratina, Rebecca L Ambrose, Marcel Doerflinger, Zhaoqing Hu, Emma Morrish, Diep Chau, Andrew J Kueh, Cheree Fitzibbon, Marc Pellegrini, Jaclyn S Pearson, Michael O Hottiger, Andrew Webb, Najoua Lalaoui, John Silke

Journal article  |  2022  |  SCIENCE ADVANCES

Tumor necrosis factor (TNF) is a key component of the innate immune response. Upon binding to its receptor, TNFR1, it promotes pro..

Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms

Christoph Grohmann, Charlene M Magtoto, Joel R Walker, Ngee Kiat Chua, Anna Gabrielyan, Mary Hall, Simon A Cobbold, Stephen Mieruszynski, Martin Brzozowski, Daniel S Simpson, Hao Dong, Bridget Dorizzi, Annette Jacobsen, Emma Morrish, Natasha Silke, James M Murphy, Joan K Heath, Andrea Testa, Chiara Maniaci, Alessio Ciulli, et al.

Journal article  |  2022  |  NATURE COMMUNICATIONS

Modulation of protein abundance using tag-Targeted Protein Degrader (tTPD) systems targeting FKBP12F36V (dTAGs) or HaloTag7 (HaloP..

The Lck inhibitor, AMG-47a, blocks necroptosis and implicates RIPK1 in signalling downstream of MLKL

Annette Jacobsen, Catia L Pierotti, Kym N Lowes, Amanda E Au, Ying Zhang, Nima Etemadi, Cheree Fitzgibbon, Wilhelmus JA Kersten, Andre L Samson, Mark F van Delft, David CS Huang, Helene Jousset Sabroux, Guillaume Lessene, John Silke, James M Murphy

Journal article  |  2022  |  CELL DEATH & DISEASE

Necroptosis is a form of caspase-independent programmed cell death that arises from disruption of cell membranes by the mixed line..

Interferon-g primes macrophages for pathogen ligand-induced killing via a caspase-8 and mitochondrial cell death pathway

Daniel S Simpson, Jiyi Pang, Ashley Weir, Isabella Y Kong, Melanie Fritsch, Maryam Rashidi, James P Cooney, Kathryn C Davidson, Mary Speir, Tirta M Djajawi, Sebastian Hughes, Liana Mackiewicz, Merle Dayton, Holly Anderton, Marcel Doerflinger, Yexuan Deng, Allan Shuai Huang, Stephanie A Conos, Hazel Tye, Seong H Chow, et al.

Journal article  |  2022  |  IMMUNITY

Cell death plays an important role during pathogen infections. Here, we report that interferon-γ (IFNγ) sensitizes macrophages to ..

Digesting the Role of JAK-STAT and Cytokine Signaling in Oral and Gastric Cancers.

Yanhong Ni, Jun T Low, John Silke, Lorraine A O'Reilly

Journal article  |  2022  |  Front Immunol

When small proteins such as cytokines bind to their associated receptors on the plasma membrane, they can activate multiple intern..