Isabelle Rouiller

MDSPACE: Extracting Continuous Conformational Landscapes from Cryo-EM Single Particle Datasets Using 3D-to-2D Flexible Fitting based on Molecular Dynamics Simulation.

Rémi Vuillemot, Alex Mirzaei, Mohamad Harastani, Ilyes Hamitouche, Léo Fréchin, Bruno P Klaholz, Osamu Miyashita, Florence Tama, Isabelle Rouiller, Slavica Jonic

Journal article  |  2023  |  J Mol Biol

This article presents an original approach for extracting atomic-resolution landscapes of continuous conformational variability of..

In silico prediction, characterization, docking studies and molecular dynamics simulation of human p97 in complex with p37 cofactor.

Abolfazl Mirzadeh, George Kobakhidze, Rémi Vuillemot, Slavica Jonic, Isabelle Rouiller

Journal article  |  2022  |  BMC Mol Cell Biol

BACKGROUND: The AAA + ATPase p97 is an essential unfoldase/segragase involved in a multitude of cellular processes. It functions a..

The AAA ATPase p97 as a novel parasite and tuberculosis drug target

George Kobakhidze, Ashish Sethi, Sepideh Valimehr, Stuart A Ralph, Isabelle Rouiller

Journal article  |  2022  |  TRENDS IN PARASITOLOGY

The multifunctional AAA+ ATPase p97 is an unfoldase/segregase involved in various cellular processes and present in all kingdoms o..

Broad and ultra-potent cross-clade neutralization of HIV-1 by a vaccine-induced CD4 binding site bovine antibody

Behnaz Heydarchi, Danielle S Fong, Hongmei Gao, Natalia A Salazar-Quiroz, Jack M Edwards, Christopher A Gonelli, Samantha Grimley, Turgut E Aktepe, Charlene Mackenzie, William J Wales, Marit J van Gils, Albert Cupo, Isabelle Rouiller, Paul R Gooley, John P Moore, Rogier W Sanders, David Montefiori, Ashish Sethi, Damian FJ Purcell

Journal article  |  2022  |  CELL REPORTS MEDICINE

Human immunodeficiency virus type 1 (HIV-1) vaccination of cows has elicited broadly neutralizing antibodies (bNAbs). In this stud..

NMMD: Efficient Cryo-EM Flexible Fitting Based on Simultaneous Normal Mode and Molecular Dynamics atomic displacements

Remi Vuillemot, Osamu Miyashita, Florence Tama, Isabelle Rouiller, Slavica Jonic

Journal article  |  2022  |  JOURNAL OF MOLECULAR BIOLOGY

Atomic models of cryo electron microscopy (cryo-EM) maps of biomolecular conformations are often obtained by flexible fitting of t..

A "Drug Sweeping'' State of the TriABC Triclosan Efflux Pump from Pseudomonas aeruginosa

Lucien Fabre, Abigail T Ntreh, Amira Yazidi, Inga Leus, Jon W Weeks, Sudipta Bhattacharyya, Jakob Ruickoldt, Isabelle Rouiller, Helen Zgurskaya, Jurgen Sygusch

Journal article  |  2021  |  STRUCTURE

The structure of the TriABC inner membrane component of the triclosan/SDS-specific efflux pump from Pseudomonas aeruginosa was det..

Plant-derived virus-like particle vaccines drive cross-presentation of influenza A hemagglutinin peptides by human monocyte-derived macrophages

Alexander Makarkov, Makan Golizeh, Elizabeth Ruiz-Lancheros, Angelica A Gopal, Ian N Costas-Cancelas, Sabrina Chierzi, Stephane Pillet, Nathalie Charland, Nathalie Landry, Isabelle Rouiller, Paul W Wiseman, Momar Ndao, Brian J Ward

Journal article  |  2019  |  NPJ VACCINES

A growing body of evidence supports the importance of T cell responses to protect against severe influenza, promote viral clearanc..

An Asymmetric Opening of HIV-1 Envelope Mediates Antibody-Dependent Cellular Cytotoxicity

Nirmin Alsahafi, Nordine Bakouche, Mohsen Kazemi, Jonathan Richard, Shilei Ding, Sudipta Bhattacharyya, Durba Das, Sai Priya Anand, Jeremie Prevost, William D Tolbert, Hong Lu, Halima Medjahed, Gabrielle Gendron-Lepage, Gloria Gabrielle Ortega Delgado, Sharon Kirk, Bruno Melillo, Walther Mothes, Joseph Sodroski, Amos B Smith, Daniel E Kaufmann, et al.

Journal article  |  2019  |  CELL HOST & MICROBE

The HIV-1 envelope glycoprotein (Env) (gp120-gp41)3 is the target for neutralizing antibodies and antibody-dependent cellular cyto..

The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution

Michael Luke Carlson, John William Young, Zhiyu Zhao, Lucien Fabre, Daniel Jun, Jianing Li, Jun Li, Harveer Singh Dhupar, Irvin Wason, Allan T Mills, J Thomas Beatty, John S Klassen, Isabelle Rouiller, Franck Duong

Journal article  |  2018  |  ELIFE

Membrane proteins are difficult to work with due to their insolubility in aqueous solution and quite often their poor stability in..

Mfn2 ubiquitination by PINK1/parkin gates the p97-dependent release of ER from mitochondria to drive mitophagy

Gian-Luca McLelland, Thomas Goiran, Wei Yi, Genevieve Dorval, Carol X Chen, Nadine D Lauinger, Andrea I Krahn, Sepideh Valimehr, Aleksander Rakovic, Isabelle Rouiller, Thomas M Durcan, Jean-Francois Trempe, Edward A Fon

Journal article  |  2018  |  ELIFE

Despite their importance as signaling hubs, the function of mitochondria-ER contact sites in mitochondrial quality control pathway..